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Product Info Summary
| SKU: | RP1057 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Occludin/OCLN Antibody
SKU/Catalog Number
RP1057
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Occludin/OCLN Antibody catalog # RP1057. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Occludin/OCLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1057)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Occludin recombinant protein (Position: N373-T522). Human Occludin shares 89% amino acid (aa) sequence identity with both mouse and rat Occludin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1057 is reactive to Ocln in Human
Applications
RP1057 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
59 kDa
Calculated molecular weight
59kDa
Background of Occludin
Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-Occludin antibody, RP1057, Western blotting
All lanes: Anti Occludin (RP1057) at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: U87 Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD
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Anti-Occludin antibody, RP1057,IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For Ocln (Source: Uniprot.org, NCBI)
Gene Name
Ocln
Full Name
Occludin
Weight
59kDa
Superfamily
ELL/occludin family
Alternative Names
BLCPMG; Occludin; OCLN Ocln|AI503564, O, Ocl|occludin|occludin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ocln, check out the Ocln Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ocln: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Occludin/OCLN Antibody (RP1057)
Hello CJ!
No publications found for RP1057
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Anti-Occludin/OCLN Antibody
Question
We are currently using anti-Occludin/OCLN antibody RP1057 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The anti-Occludin/OCLN antibody (RP1057) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-29
Question
Is this RP1057 anti-Occludin/OCLN antibody reactive to the isotypes of OCLN?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The immunogen of RP1057 anti-Occludin/OCLN antibody is E.coli-derived human Occludin recombinant protein (Position: N373-T522). Human Occludin shares 89% amino acid (aa) sequence identity with both mouse and rat Occludin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-10
Question
We have been able to see staining in human liver. Do you have any suggestions? Is anti-Occludin/OCLN antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
From literature liver does express OCLN. From Uniprot.org, OCLN is expressed in thyroid gland, colon carcinoma, liver, brain lung, embryonic kidney, among other tissues. Regarding which tissues have OCLN expression, here are a few articles citing expression in various tissues:
Brain, and Lung, Pubmed ID: 15489334
Colon carcinoma, Pubmed ID: 8601611
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 9175707, 18318008, 20463075, 24275569
Boster Scientific Support
Answered: 2019-12-10
Question
I was wanting to use your anti-Occludin/OCLN antibody for WB for human embryonic kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
As indicated on the product datasheet, RP1057 anti-Occludin/OCLN antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-08
Question
Our lab want to know about to test anti-Occludin/OCLN antibody RP1057 on human embryonic kidney for research purposes, then I may be interested in using anti-Occludin/OCLN antibody RP1057 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The products we sell, including anti-Occludin/OCLN antibody RP1057, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-22
Question
We want using your anti-Occludin/OCLN antibody for bicellular tight junction assembly studies. Has this antibody been tested with western blotting on sw620 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
I appreciate your inquiry. This RP1057 anti-Occludin/OCLN antibody is validated on sw620 whole cell lysate, colo320 whole cell lysate, a549 whole cell lysate, 293t whole cell lysate, u87 whole cell lysate, mammary cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-24
Question
My colleagues were happy with the WB result of your anti-Occludin/OCLN antibody. However we have observed positive staining in colon carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is OCLN supposed to be expressed?
T. Wu
Verified customer
Asked: 2019-04-17
Answer
From literature, colon carcinoma does express OCLN. Generally OCLN expresses in cell membrane. Regarding which tissues have OCLN expression, here are a few articles citing expression in various tissues:
Brain, and Lung, Pubmed ID: 15489334
Colon carcinoma, Pubmed ID: 8601611
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 9175707, 18318008, 20463075, 24275569
Boster Scientific Support
Answered: 2019-04-17
Question
We bought anti-Occludin/OCLN antibody for IHC on thyroid gland in a previous project. I am using human, and We are going to use the antibody for WB next. My lab would like examining thyroid gland as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-06-08
Answer
I looked at the website and datasheets of our anti-Occludin/OCLN antibody and I see that RP1057 has been tested on human in both IHC and WB. Thus RP1057 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-08
